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Oligonucleotides defining the binding site were labeled with Alexa Fluor 488 dye and mixed with unlabeled RaaS.
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The conserved LENRV motif is indicated with a double line and a NIMIN binding site is labeled with.
The binding site was labeled with the label of the closest gene that was part of an expression cluster.
After antibody incubation, the binding sites were labelled with Dako Envision + polymer linked secondary reagent and visualized using diaminobenzidine (DakoCytomation).
Mutations of hydrophobic residues in the three AS binding sites are labeled.
For each comparison, a group of microenvironments in the query site that match counterparts in one drug-binding site are labeled as a "druggable subsite".
For electrophoretic mobility shift assays (EMSAs), synthetic oligonucleotides containing the putative CTCF binding site were 5′-end labelled by γ-P-ATP and subsequently purified by gel filtration on Sephadex G-25 Medium columns.
BubR1 residues essential for B56 binding are labelled with red stars, whereas phosphorylation sites are labeled green pound signs.
Double-stranded oligonucleotides containing indicated homeodomain and FoxD binding sites were radioactively labeled at the 5'ends with γ32PdATP using polynucleotide kinase (Boehringer Manheim) and purified on microspin columns (Amersham Biosciences).
Examination of the first site, between trnE and trnF, where primary transcripts labeled A and D in Figure 1 meet, show that this binding site is not completely conserved across Diptera and is absent from the Mecoptera (Fig. 2 A ).
From the 16,830 binding sites that are labeled as Good by either of the default profiles, 85% of them are identified by the Default (PDB_REDO) profile [in contrast with only 46% being identified by the Default (PDB) profile].
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CEO of Professional Science Editing for Scientists @ prosciediting.com