Sentence examples for binding site were designed from inspiring English sources

Putative FOXM1 DNA binding sites [67] within promoter regions (within 1 Kb upstream of gene) of CEP55 and HELLS were identified using ClustalW2 sequence alignment tool [68]) and qPCR primers flanking the putative binding site were designed (Supplemental Fig. S9).

Specific primers for each binding site were designed (primer information is available upon request from the corresponding author).

In this study, based on the anti-proliferative activities, a novel tubulin inhibitor 7a3 targeting the colchicine binding site was designed, synthesized, and optimized from a series of novel cis-restricted pyrazole analogues of combretastatin A-4.

Based on NN703, low molecular weight growth hormone secretagouges (GHSs) with a reduced number of hydrogen binding sites were designed by removal of the C-terminal amide group.

Simple and efficient sensors 1 and 2 possessing azo and nitrophenyl as signaling units and urea and thiourea moieties as binding sites were designed and synthesized.

For several genes, multiple putative promoters were identified by the program (up to 4 for ARRDC3) and primers surrounding the putative forkhead binding sites were designed.

The binding sites were designed to allow incomplete base pairing with miR-312, such that the 5'seeded' and 3' regions of the miRNA would be fully base paired, with a central unpaired bulge (Fig. 1D).

The oligonucleotides flanking transcription start site and predicted Tcf4 binding sites were designed within ~200-bp windows in the 700-bp upstream of the transcription start site (amplicon U), and in the 500-bp windows downstream of transcription start site (amplicon D) for detecting miR-181a-2 miR-181a-2 miR-181a-2er.

To confirm the presence of a putative binding site for miR-24-2 withen the 3'UTR region of the BCL-2 gene, the specific primers flanking the binding sites were designed and the resulting amplicon was cloned into the 3'UTR region of the luciferase gene of the reporter vector pGL3 (pGL3/ BCL-2).

With the aim to discover a gastric antisecretory agent more potent than the existing proton pump inhibitors, novel 3,4-dihydro-1H-spiro naphthalene-2,2′-piperidin -1-one derivatives, which could occupy two important lipophilic pockets (described as LP-1 and LP-2) of H+,K+-ATPase and can strongly bind to the K+-binding site, were designed based on a docking model.

Briefly, the primers for p65 protein-binding site were designed as: forward: 5′-GCTTAGAAAGGTAGAGGGTG-3′, reverse: 5′-ACTGACTCTCCTGCTCTGCT-3′.