Sentence examples for binding site we performed from inspiring English sources

To determine whether γ-tubulin, similar to Dp1 (1), binds to the E2F DNA binding site, we performed an electrophoretic mobility shift assay (EMSA; ref. 31).

In order to test if the K5 Hs II segment contains a bona fide p63 binding site, we performed Electrophoretic Mobility Shift Assays (EMSA).

To test the functional consequence on enhancer blocking by the mutated binding site, we performed transfection experiments with episomes containing the F1F2 or the F1mutF2 binding sites.

To determine whether Tat expression affects the in vitro binding of AP-1 factors to each individual intragenic AP-1 binding site, we performed EMSAs with nuclear extracts from mock-treated or PMA-treated HeLa cells expressing the one-exon form of Tat (pTat72, see the Materials and Methods section), or the two-exon form of Tat (pTat101) (Figure 6A).

To determine the lncRNA binding site, we performed alanine scanning mutagenesis of WDR5, guided by its crystal structure (Trievel and Shilatifard, 2009; Odho et al., 2010; Avdic et al., 2011).

To explore the interactions of GABA in the binding site, we performed molecular dynamic (MD) simulations of a homology model of the GABAC receptor ECD in water to validate the homology model and assess the interactions of GABA inside the alleged binding site.

To determine DNA-binding motif(s) that was enriched at the Atf1/Pcr1 binding sites, we performed the motif-discovery scan [44] using the 150bps sequence located at the apexes (see Materials and Methods).

Since the IGF-2 gene promoter contains multiple consensus STAT binding sites, we performed electromobility shift assays with nuclear extracts of untreated or PRL-treated T37i cells and the STAT response element located −3184 bp upstream of the transcription initiation start site of IGF-2 gene (exon 1) (Fig. 4F).

In order to gain insight into the mechanism of how cohesin is recruited to the ESC transcription factor binding sites, we performed a proteomic study on Nanog, the transcription factor with the highest enrichment score and the most similar expression pattern changes upon RAD21 depletion.

To investigate the sequencing depth necessary to saturate the number of detectable binding sites, we performed the following computational simulation.

To identify the critical binding sites, we performed a comprehensive binding assay that utilized mutants containing four random amino acid mutations spanning nearly all of the loop regions.