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Scanning the whole protein without using any prior knowledge of the binding site, we find that the best scoring conformation in rhodopsin is 1.1 Å CRMS from the crystal structure for the ligand atoms.
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Amino acid variations at binding site were found to be more than other part of alleles.
When several known factor Xa inhibitors were overlaid within the protein binding site, we found that they all bind within the groove formed by the S1 and S4 sub-pocket of fXa (Figure 10B).
Site1, the binding site we found by DS2.1 automatically searching, can cover the structural domain included by endogenous ligand.
Interestingly, using a reporter system containing Nanog response element (binding site), we found that Li significantly enhanced the expression of reporter in HEK293T cells co-expressed with Nanog.
While the human tetherin cytoplasmic tail contains a predicted TRAF6 binding site, we found that this interaction was actually determined by the adjacent endocytic motif YDYCRV.
Other novel motifs were also found, but these were all accompanied by a conserved motif in the other binding site, we found RKVA and RRLW in BM1, and REYL and RRFL in BM2.
Assessing the content of the established consensus sequence for STAT1 binding sites, we find that the usage of "negative control" ChIP-seq data fails to provide substantial advantages.
Based on over-representation analysis of their binding sites we find that the expression pattern for the genes of these six transcription factors correlate nicely with the time profiles of their putative target gene clusters.
Using chromatin immunoprecipitation (ChIP) and primers designed to flank these YB-1 putative binding sites, we found evidence that YB-1 binds to the SOX2 promoter at two adjacent regions.
Among GR binding sites, we found that 46%% occur at enhancers having all three marks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com