The chvE homologs of 1021 and OV14 are ~100 bp shorter than the version located in C58, with the difference found towards the N terminus of the gene where a putative ligand binding site is positioned.
In Saccaromyces cerevisiae, transcription factor binding sites are positioned further upstream in the intergenic regions and vary over a wide range in promoters [ 20].
However, how these binding sites are positioned throughout the three-dimensional space inside the nucleus was unknown, as was the likelihood of Sox2 finding and binding to these sites.
Given that three MRE binding sites are positioned within 299 bp of the proximal wMT-2 promoter [21], we tested their binding capacity by means of EMSA using cytosolic and nuclear protein extracts from control and Cd-exposed earthworms.
Unexpectedly we find that BanLec adopts a tetrameric stoichiometry in solution whereby the glycan-binding sites are positioned to optimally target glycosylated viral spikes.
The primer-binding sites were positioned to be conserved across the 127 complete mt genomes in the dataset of Pierson et al. [ 11].
Thus, it was interesting to determine how candidate human-specific TF-binding sites are positioned in the genome relative to the genomic coordinates of these 24 genes.
In this study, a highly conserved canonical C/EBP α binding site was located around position −45~−54 bp relative to the human FTO gene transcriptional start site.
In the same crystal structure, a similar ligand binding site was also observed in the symmetric position in CTM.
A putative small-ligand binding site was located within each repeat in a position analogous to the serine-binding site of the ACT-domain of the Escherichia coli phosphoglycerate dehydrogenase.
