Sentence examples for binding site was mutated from inspiring English sources

This inhibition response was abolished when miR-29 binding site was mutated, since this mutation (pLuc-3URTmutant-p85) prevents miR-29 binding to targets in the mRNA.

Luciferase reporters were constructed containing either wild-type full-length Gli1 3′UTR (pMIR/Gli1) or a mutated Gli1 3′UTR (pMin/Gli1/mut, in which the sequence of the putative miR-133b binding site was mutated).

On the contrary, we observed bimodality (Figure 4D) when the SigA binding site was mutated.

When the 124/506B binding site was mutated, miR-124 still upregulated lucifease expression to 240% (p = 0.0030).

When the 148/152A binding site was mutated, miR-148 downregulated reporter gene expression to 47% (p = 0.0049).

When the 124/506A binding site was mutated, miR-124 no longer affected expression of the reporter gene.

Three nucleotides in the core sequence of putative NF-κB binding site were mutated (GGG changed to CTC, Fig. 2B).

Several residues lining the putative binding acyl chain binding site were mutated to purturb substrate binding.

Residues N71, Q104, and S192, which are located around the Pc binding site, were mutated to cysteine.

This could be achieved if the Cbf-K activator binding site is mutated resulting in low-level to no increase in transcription of the Cbf-K gene.

To determine whether miR-146a inhibits Smad4 expression through this seed sequence, we constructed luciferase reporter plasmids harboring the wildtype 3' UTR and the mutant 3' UTR in which the putative miR-146a binding site is mutated.