Sentence examples for binding site was generated from inspiring English sources
The mutant construct Luc-ADCY5-mut, mutation of the miR-17-5p binding site, was generated by PCR approach with primers musADCY5-SacI and musADCY5-MluI-mut (5′-CCACGCGTCAGAAGTTGCTTCTGAGTGTTAGTG-3′).
The reporter construct with mutated Sox10 binding site was generated using QuickChangeII Site-directed Mutandnesis Kit and a pair of mutagenizing oligonucleotide primers (5'-GTTTGAAGAGGCAGACTGGTCTCTTTATTG-3', 5'-CAATAAAGAGACCAGTCTGCCTCTTCAAAC-3').
HNF4G mutant (mut) expression vector (without miR-34a specific 3′-UTR binding site) was generated with the QuikChange Multi Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer's protocol.
The plasmid of pCMA-HAOCT4-3′UTRmu encoding a mutation of HA-human OCT4, including 3′UTR (deleted the miR-34a binding site), was generated using pCMA-HAOCT4-3′UTR as a template with proper primers (Supplementary Table S2).
The 'seed' deleted 3′UTR of CCL4 lacking of 7-nt of the binding site was generated from the WT reporter plasmid with primers (forward: 5′-tttctcgagCCAAAAGAAGCAAGC-3′ and backward: 5′-gagtggtgaccTAGACTTCCTGTCTCTGAGCAGC-3′).
We chose the mutations with the help of the bioinformatics predictions of the TTF-1 binding consensus sequence such that on the CNS87 element TTF-1 binding was totally abrogated in the FT-1 and FT-6 regions and no other TTF-1 binding site was generated within the entire element.
Promotor region segments of human collectrin containing putative HNF-1 binding site were generated by PCR and cloned into pGL3 basic reporter vector (Promega)[13].
Oligonucleotides corresponding to Tcf21 promoter with or without SRY binding site were generated by PCR amplification with primers listed in the Table S3.
Through this sort of artificial arrangement, the pyruvate binding site is generated that would not be present in solution [10], This allowed us to solve the structure of OcDH/NADH-pyruvate which in solution is not observed.
A background sequence for a particular binding site is generated of length equal to that of its upstream region (up to 600 bps).
A dataset of ligand-dependent binding sites was generated using the SiteHopper create tool [13] where default parameters create a binding site patch within 4 Å of a specified bound ligand.
