Sentence examples for binding site was constructed from inspiring English sources
18 Using the same approach, a model of the 5-HT3AB receptor binding site was constructed by homology modeling using MOE (version 2010.10, Chemical Computing Group, Montreal).
The BRCA1-W and -M CDS of miR-638 binding site was constructed in pGL3 plasmid (a gift from Dr. Wen Chen from Sun Yat-sen University, China).
N-terminal duRIG-I, in which residues 1 244 encode the tandem CARDs alone, without the ATP binding site, was constructed.
The plasmid of psiCHECKOCT4-3′UTR encOCT4g orT4 3′UTR or psiCHECKOCT4-3′UTR-DM encoding a mutation of OCT4 3′UTR (deleted the miR-34a binding site) was constructed using plasmid pCMA-HAOCT4-3′UTR as a template for PCR with proper primers (Supplementary Table S2).
A non-enzymatic electrochemiluminescence (ECL) sensor based on the competitive reaction between glucose and phenoxy dextran (DexP) for concanavalin A (ConA) binding sites was constructed for detecting glucose.
The promoter region of Cricetulus griseus HMG synthase from −368 to +1 bp, containing SREBP binding sites, was constructed by Eurofins (Germany) with SmaI and XhoI restriction sites.
A position weight matrix (PWM) representing ribosome binding sites was constructed by running ELPH [ 81] on the 25 bp regions upstream of the predicted start sites.
To further confirm the direct regulation of FOXO1 by miR-1269, the pGL3- FOXO1-3'-UTR-luciferase reporter, containing the three putative miR-1269 binding sites, was constructed.
Human CYP2E1 luciferase reporter containing putative p53 binding sites was constructed by amplifying the upstream region of the CYP2E1 promoter -7873 to -5896 (counted from the translation initiation site) and inserting it in the pGL3 promoter luciferase vector (Promega, Madison, WI, USA).
To further verify this specific binding, a mutated pMIR-TLR3 vector with a three-nucleotide mutation in the putative seed-binding site was constructed and transfected together with miR-26a mimics and pRL-TK into Hela cells.
Besides, mutant vectors containing four mutated bases on the predicted binding sites were constructed.
