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Comparison of the rat, human and mouse collectrin genes using GenBank data revealed that HNF-1 binding site was conserved.
The binding site was conserved among human, mouse and rat.
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In DARPins that have been selected to bind to a designated target, the residues that do not form the binding site are conserved, whereas the residues that form the binding site are derived from randomised sequences.
Furthermore, an Rbpjκ binding site is conserved on the promoters of Nrf2 among animal species.
All nucleotides of the seed sequence of the binding site are conserved in several species, including human, mouse and rat.
The β-1,3 Gal binding site is conserved with the other three GI NoVs, while the Le epitope binding sites are particularly well developed, formed by five residues and interact with Le epitope via six hydrogen (H) bonds, highlighting the importance of the Le epitope binding site in BV.
Further analysis of this binding site reveals that the YopJ binding site is conserved in other MKKs.
The EuphMLSUF primer binding site is conserved in the euphausiid genera we were targeting and is not conserved in sequences available from non-euphausiid crustaceans (Table S1).
All positions are not highly conserved; however the hydrophobic nature of the binding site is conserved.
The APC/C binding site is conserved in all mammals with the exception of Cavia porcellus.
Within this group, the three acidic residues in the putrescine binding site are conserved.
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