Sentence examples for binding site was blocked from inspiring English sources

Nonspecific binding site was blocked with 5% normal goat serum containing permeabilisation buffer for 30 minutes.

Unspecific binding site was blocked with 5% normal horse serum in PBST for at least one hour at room temperature.

After rinsing the sections in phosphate-buffered saline (PBS, pH 7.4), the nonspecific binding site was blocked with 10% normal goat serum for 20 minutes at room temperature.

The nonspecific antibody binding site was blocked by using 3% BSA in PBS-T for 1 h, followed by 1 h incubation with goat anti-mouse Pyk2 and paxillin.

Because, in some models, the binding site was blocked by rotamers of a residue in close proximity to the binding site, we excluded models with a particularly small volume by selecting 10 models with the largest calculated binding site volume as the docking receptors.

The crystal structure of FST288:activin complex has shown that the type II receptor binding site is blocked by both FSD1 and FSD2 (Thompson et al. 2005).

Quite often, access to the ribosome binding site is blocked by sRNA-mRNA base pairing.

The residual binding sites were blocked through incubation in TBS containing 0.2% Tween and 5% BSA.

Unspecific binding sites were blocked by incubating membranes in phosphate-buffered saline containing 0.5%Tween-20Tween-20and5% or bovine serum albumin.

After washing, unoccupied binding sites were blocked with blocking buffer (0.1 M Tris-HCl, pH 8.0) at room temperature for 2 h.

Nonspecific binding sites were blocked by incubating with 5% non-fat milk, and then the membranes were incubated with primary antibodies.