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A binding site was assigned as soon as it matched the pattern.
When only one gene was located in an appropriate orientation, the binding site was assigned to this gene.
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Each of the top 2,000 emergent MLL4 binding sites was assigned to its closest transcription start sites (TSS).
One obvious limitation of our approach is the fact that for the purpose of the aforementioned studies, a specific p63-binding site was assigned to be regulatory element for the nearest gene, often without any supporting experimental data.
Sequence positions except the binding sites are assigned by searching for a base sequence that will fold into the target structure designed in the previous step using RNAinverse[28] or RNAdesigner from RNAsoft[31], with a non-binding pseudo-base (N) being assigned to all positions in the binding region.
Except for one sequence all possible binding sites were assigned to non-coding regions.
Therefore, 146 major Atf1/Pcr1 binding sites were assigned to the probable promoter of the total of 158 genes.
In particular, all the annotated (resp. non-annotated) binding sites are assigned a higher (resp. lower) binding probability than without conservation data.
The external dataset defined by Hajduk et al. [27] contains 72 proteins, of which 35 binding sites were assigned as druggable and the remaining 37 sites as non-druggable, based on whether high-affinity druglike binders could be found in the literature.
All LRH-1 binding sites were assigned to nearest genes based on the Mus musculus NCBI m37 genome assembly (mm9; July 2007).
Identified p53-binding sites were annotated and the locations of p53-binding sites were assigned to RefSeq genes [22].
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