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The association between the presence of L1 and AGO2 binding site was analyzed using chi-square test.
The ATP binding site was analyzed using the Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB) (http://www.pdb.org) [ 38].
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To determine whether a gene was also a predicted target of miR-200b and c, the presence of miR-200 family binding sites was analyzed using TargetScan 5.0 (www.targetscan.org [56]).
Integrity of transcription factor binding sites was analyzed by comparing the SNP distributions within and outside the putative transcription binding sites.
The correlation between deregulated genes in coInsm1 mutant islets and various categories of binding sites was analyzed, and the best correlation was found for Insm1/Neurod1/Foxa2 sites.
527 bp of sequence (excluding 38 nucleotides at each end of the amplicon, corresponding to the primer binding sites) was analyzed for the phylogenetic tree construction by the neighbor-joining method using the MEGA 5.05 package (http://www.megasoftware.net/mega.php).net/mega.php
Using the structures of ODC and AZI, the solvent accessibility of the putative AZ-binding site was analyzed (http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html, [50]).
A total of 37 CpG sites containing many overlapping transcription factor binding sites were analyzed for site-specific methylation status using bisulfite methylation sequencing analysis.
The miRNA binding sites were analyzed, and the miRNAs mmu-let-7a and mmu-mir-202-3p mmu-mir-202-3p mmu-mir-202-3p mmu-mir-202-3p binding and good dowereream binding to their targets in predictedand Ppargc1b (Fig. 5a).
Later, miR390 conserved binding sites were analyzed on EST candidates.
Promoter sites and potential transcription factor binding sites were analyzed by Genomatix software suite (Genomatix Software GmbH, Munich, Germany).
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