Sentence examples for binding site using the from inspiring English sources
Namely, first we compute non-equilibrium works generated during pulling ligands from the binding site using the steered molecular dynamics method.
In this work, we have docked this series of peptidomimetics into the TR binding site, using the FlexX algorithm as implemented in the Sybyl program, in order to access the binding mode of this class of compounds in the target enzyme.
The mutated version of this plasmid (p-zbtb7a 3'UTRm) was generated by utilizing the p-zbtb7a 3'UTR as template and modifying the miR-20a seed binding site using the QuikChange II XL Site-Directed Mutagenesis Kit.
Mutagenesis was used to delete miR-17 binding site using the QuickChange II kit (Stratagene).
Mutagenesis was used to delete miR-17 binding site using the QuickChange II Kit (Stratagene, La Jolla, CA, USA).
We generated 2D schematic representations of the ligands in the binding site using the LIGX option in MOE (Chemical Computing Group, Montreal, Quebec, Canada).
The UCH L1 promoter was mutated at different TCF4/Lef1 binding sites using the Quik-Change Site Directed Mutagenesis Kit (Stratagene).
DNA sequences in the promoter region were analysed with the TESS interface (http//:www.cbil.upenn.edu/tess) for putative transcription factors binding sites using the TRANSFAC database.
However, we envision that it is feasible to increase the selectivity of bioinformatics searches by modeling higher order cis-regulatory modules (CRMs) [31], consisting of several transcription factor binding sites, using the same HMM statistical principles and software described herein.
We searched for both known transcription factor binding sites using the Transfac database (http://www.cbil.upenn.edu/cgi-bin/tess/tess?RQ=NBqt) and for enrichment of novel sequence motifs.
