The importance of these binding sites to the expression of the CAMP gene remains to be fully elucidated.
ChIP-seq experiments to determine whether or not most of these overrepresented motifs act as true E2F binding sites will help to elucidate the importance of E2F transcription factors in C. parvum transcriptional regulation.
Recently a C-terminal binding site has been elucidated and a structure bound with novobiocin modeled (Fig. 7a b) [83].
Potential binding sites were elucidated by docking.
C/EBP binding sites were elucidated by using a web-based search tool on http://www.cbrc.jp/research/db/TFSEARCH.html.
The basic strategy of iterated HMM should be applicable in predicting dif analogues in these species when defined seed sequences and detailed positions of recombinase binding sites are elucidated.
Further work to identify the DNA-binding protein that binds to this site will help to elucidate the mechanistic basis of how variants within the MLH1 5′UTR affect expression.
In this study, we used molecular dynamics, radioligand binding, and thermostability experiments to elucidate the role of the recently discovered sodium ion binding site in the allosteric modulation of the human A2A adenosine receptor, conserved among class A GPCRs.
Additional studies to elucidate binding sites for the remaining putative C. parvum transcription factors and DNA-binding proteins coupled with experiments to determine their binding sites throughout the genome (ie, utilizing ChIP-seq) are needed to distinguish between these possibilities.
To study the binding mode in the active site of plasmodial ODC and to elucidate binding differences, PPT3 and PPT5 were modeled into the active site of PfODC (Figure 2).
