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Docking identified a binding site that matched the location of the NMBD in our previous structure by cryo-electron microscopy, allowing a more detailed view of its binding configuration and further support for its role in autoinhibition.
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To investigate whether PAX3-FKHR controls CPT1A transcription by binding to CPT1A's promoter, we analyzed the DNA sequence 5′ to the CPT1A transcription start site and identified 3 putative PAX3-FKHR binding sites that matched the consensus composite sequence: a paired-box domain (PD) binding site (GTTC/AT/C) followed by a linker sequence and a homeodomain (HD) binding site (ATTA).
In this respect, the terR promoter contains two putative AreA binding sites that match the HGATAR consensus (BS1and BS2; positions −59 and −72 relative to the translational start point).
For each comparison, a group of microenvironments in the query site that match counterparts in one drug-binding site are labeled as a "druggable subsite".
MED-SuMo uses the binding site of the target as a query to search either the whole PDB (or any corporate protein structure databank) for all the binding sites that display a local match with the query.
We find that human and chimp FOXP2 have similar binding sites that are distinct from previously suggested consensus binding sites.
Most other significantly enriched sites corresponded to binding sites for transcription factors from several families, in addition to another CTCF binding site that in fact matches the composition of the M2 moiety of the full CTCF binding site (Schmidt et al. 2012).
Input sequences were aligned and regions closely matching known TF-binding sites that were conserved in more than 50% of the input sequences (4 out of 8) were mapped.
The SRF binding site also matched to the same motif identified as a MEF2 site (a1).
Highly selective ion discrimination should occur when binding sites form rigid cavities that match K+, but not the smaller Na+, ion size or when binding sites are composed of specific chemical groups.
Six potential κB binding sites were identified that matched the consensus κB binding sequence.
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