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c A relatively large bioactive compound (purple sticks) forms hydrogen bonds with Asp415 in the binding site prepared with Maestro.
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JB conducted alternative splicing experiments and bioinformatic analysis; MP conducted in situ hybridization experiments; RD identified transcription factor binding sites; AF prepared text and figures, and assisted with data analysis; AR supervised studies and assisted with data analysis.
Based on these 12 predicted binding sites, we prepared 11 probes for EMSAs (see Fig. S10 and Table S4).
ND analysed the TRANSFAC binding sites and prepared the data sets.
Electrode sites were prepared with highly conductive gel.
To identify native TR-binding sites, we prepared chromatin from the TRα1 expressing tissues brain and heart of TRα1 GFP mice, and performed ChIP with an anti-GFP antibody.
Moreover, Surflex-PSIM [34], a novel method combining initial binding site recognition with subsequent binding site comparisons has been developed.
Only binding site matches with >90% identity to the core binding site motif are reported here.
The operation site was shaved and prepared with povidone-iodine.
For the intrinsic binding mode of Gd@C82(OH)22, the WW domain was prepared with multiple Gd@C82(OH)22 to facilitate the binding site search on the protein.
The MIP microspheres prepared via RAFTPP have proven to show improved binding capacity, larger binding constant and apparent maximum number for high-affinity sites, and significantly higher high-affinity binding site density in comparison with the MIP prepared via TRPP.
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