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Nevertheless, the structure of the unbound IgE scFv M0418 demonstrates that a human allergen-specific antigen binding site may display a protruding CDRH3, similar to human antibodies of other isotypes 60– 60.
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A lost binding site may have been replaced with a new, species-specific binding site - i.e. binding site turnover.
Placing a fluorophore too close to the binding site may disrupt binding, and fluorophores too far away from the binding site may not result in fluorescence changes.
Therefore, true binding sites may have SNPs less frequently than the non-binding sites.
Unoccupied 'perfect' STAT1 binding sites may include cell type specific binding sites and suggest a required but not sufficient function of the nucleotide sequence for DNA-protein binding.
These data suggest that additional binding sites may exist.
miRNA binding sites may correspond to 3 open reading frames.
Not all binding sites may evolve under the same constraints.
Since no presumptions about localization of binding sites prior to comparison are used, ProBiS may detect new binding sites and suggest ligands that these binding sites may accommodate.
Nevertheless, the RNA-binding site may also be distinct from the enzymatic site.
The nucleotide sequences of observed binding sites generally display a considerable variation, which may cause difficulties in the description of binding preferences using sequence motifs.
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