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It is interesting that the phospholipid binding site mapped by these authors overlaps the E-Syt2 binding site mapped here.
Notably, the EAT domain in Atg1 also comprises the Atg13 binding site mapped by Yeh et al., indicating that the C terminus of Atg1 mediates both membrane recruitment and Atg13 interaction in yeast.
For fmgA, a ChIP-seq peak was centered approximately 200 bp upstream of the MrpC2 binding site mapped by DNase I footprinting, which was centered at -61.5 relative to the fmgA transcriptional start site [ 46].
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The genomic loci of AR binding sites mapped by ChIP in LNCaP cells [ 4] were uploaded onto the UCSC genome browser.
To determine if there was a relationship between CTCF binding and nuclear scaffold attachment, we analyzed CTCF-binding sites mapped in HeLa cells by chromatin immunoprecipitation and high-throughput sequencing available on the UCSC Genome Browser.
The following dataset was generated: L Li, L Zhe, 2014, High-resolution Sox2 DNA-binding sites mapping by ChIP-exo in TSA-treated ES cells, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE62972, Publicly Available at NCBI Gene Expression Omnibus.
In constructing a workflow to map the binding sites of the currently characterized structural proteome, we adopted a modular approach that comprises objective binding site detection, binding site comparison (BSC), mapping of detected binding sites using unsupervised learning methods, and visualization of binding site maps.
Sox2 stable binding sites were mapped by low-excitation 2D single molecule imaging condition (Video 7 ).
In addition, 1,596 CLASH targets coincided with high-confidence AGO1-4-binding sites previously mapped by PAR-CLIP in HEK293 cells (Hafner et al., 2010a), a 3-fold enrichment over expected chance levels for expression-matched transcripts (Table S4A).
The nucleotide modifications (by DMS), the pauses corresponding to nuclease (TI and VI) cleavage sites and the toe prints corresponding to PTB binding sites were mapped to MFOLD predicted structure for clarity (Fig. 3).
The TF binding sites are mapped onto the mm8 (February 2006) mouse assembly.
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