In this case, the Br− binding site (site Br2) is shifted away from the heme iron, and site Br1 instead contains a water molecule.
For proteins where a full chemical shift assignment is available, the binding site is readily mapped from the chemical shifts of the NOE cross-peaks.
Our method for mapping the binding site is general and does not require chemical shift assignment of the enzyme resonances.
An electrophoretic mobility shift assay confirmed that an oligonucleotide containing the binding site is recognized by DBP, but a point mutant version is not (Figure 7B).
NMR chemical shift mapping has shown that levosimendan's primary binding site is also located in the N domain of cTnC in the presence of cTnI (13 , 18; however, the exact binding geometry has not been established.
Otherwise, this binding site is regarded as an incorrectly predicted binding site.
Aggregation plot revealed that the peak of FLAG-Nup98-HoxA9 binding site was severely diminished but not shifted by LMB treatment.
We usually allow a binding site to be shifted by two bases to its left and right, respectively.
In these experiments, the effects of single base pair mutations of the a1- α2 consensus binding site were measured by assaying their ability to repress transcription of a heterologous promoter and by electrophoretic mobility shift DNA-binding assays (EMSA).
No shift was observed in the control lane corresponding to the reaction mixture containing an unrelated transcriptional regulator VicR (whose binding site was not present in the murMN-SMU_718 promoter) confirming the absence of non-specific binding.
