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The objective of this study was to organize bio-receptors on surfaces in a way that their analyte binding site is exposed to the analyte solution.
The gp120 coreceptor binding site is exposed transiently, allowing gp120 attachment to the CCR5 or CXCR4 chemokine receptor (Maddon et al., 1986; McDougal et al., 1986; Rizzuto et al., 1998).
At low iron concentrations, the iron-sulfur cluster is labile and disassembled, and the RNA binding site is exposed at the IRP, enabling RNA-protein interactions [ 10].
Several lines of evidence suggest that these substitutions altered the protein's dynamics and/or increased the relative occupancy of a conformation in which the latent binding site is exposed for peptide binding.
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A small-angle X-ray scattering study of intact LLY reveals the molecule is flat and elongated with the lectin domain oriented so that the Lewis antigen-binding site is exposed.
The nucleosomes appear to be positioned in vivo in such a way that functional binding sites are exposed through a relaxed, open chromatin structure, readily accessible to any transcription factor.
In our trimer model, the front receptor-binding site was exposed at the upper surface of the Env tip and the back site at its upper periphery.
The chaperonin substrate-binding sites are exposed, and the lid is open in both the ATP-free and ATP-bound prehydrolysis states.
When proteins adsorb to the nonfreezing water layer on polymer surfaces such as PET and PHEMA, a strong conformational change of the adsorbed proteins occurs, and many cell-binding sites are exposed.
Calcium binding to troponin releases TnI from its binding site on actin, and the Tm relaxes back to its preferred position on the actin surface (C-state) where most of the myosin binding site is now exposed.
The UDP-Ara4N binding site is more solvent exposed than the PLP site.
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