This observation can be explained by the fact that since the structure of the analogue molecule is not complementary to the imprinted cavities in the MIPs produced by the glucose molecular imprinting, the ability of fructose interaction with the binding sites is expected to be weaker than that of glucose which leads to low binding capacity.
Structural independence of binding sites is expected, in particular, for proteins with multiple binding sites located on different protein domains.
24 A total of 12 ligands binding sites is expected for a complete USP domain (PF00582) following that of Methanococcus jannaschii MJ0577 protein.
Such extra density, which sits on one side of the Crm1 dimer between the two NES binding sites, is expected to correspond to bound Rev-RRE.
The observation that a significant fraction of [H]-BH4 remained bound after 24 h incubation with excess unlabeled BH4 or BH2 at 8 and 25 °C is intriguing, considering that only half of the pterin binding sites is expected to exhibit tight binding.
It should be noted that the false positive rate of 9.2% for the predicted miRNA target sites could weaken but not bias our results, because the selection pressure on the falsely predicted miRNA binding sites is expected to be same as that on their flanking regions Population genetics is a useful tool to detect the evolutionary selection on miRNA target loci [ 13].
The second relies on the biological assumption that while a TF pair plays the same role in regulating gene expression between closely related species, the occurrence of its binding sites is expected to be more highly enriched in promoter sequences of orthologous genes than in promoter sequences of non-orthologous genes.
Identification of the high-affinity ABA-binding site is expected to allow the design of ABA agonists/antagonists, which will help to understand the role of the ABA/LANCL2 system in human physiology and disease.
This gives indications of the robustness of the methods, as the biologically relevant binding sites are expected to be detected in each replicate.
These peptide-binding sites were expected to have lower levels of conservation because it is well established that the extracellular domains of GPCRs have a large degree of sequence variability.
