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Nuclear magnetic resonance studies of recombinant soluble Ii have shown that the MHCII binding site is contained in a disordered region of Ii [39] that acquires an ordered conformation upon MHCII binding [19].
The smallest fragment that labeled with both anti-FLAG antibody and 8-azido-ATP was ∼15 KDa, which suggests that the binding site is contained within amino acids 411 548 of SVOP.
In this choice we are guided by the finding [9] that most of the probability for encountering a TF binding site is contained within a window of 250 base pairs (bps) located approximately 100 bps upstream of a gene.
Our results suggest that an ssDNA binding site is contained within the OB-fold (978−1078) domain (we were unable to purify the isolated OB-domain).
The use of MCT1/MCT4 chimaeric transporters reveals that this binding site is contained within TM (transmembrane) helices 7 10 of MCT1.
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The Fab2 binding site is contains a few solvent molecules and a glycerol (GOL) molecule, which is weakly held in place by a single water-mediated hydrogen bond.
Motif finding for ZNF274 is complicated by the fact that the binding sites are contained with the final coding exon of the C2H2 target genes.
These data correlate well with the potential TIEG1 binding sites indentified in the Runx2 promoter as shown in Figure 5A since 10 of the 13 putative TIEG1 binding sites are contained within the −288 fragment and only 2 remain in the −92 fragment.
A first observation is that roughly 75% of individual binding sites are contained in CRERs, a percentage that is constant across regions (upstream, intron and 5' UTR), with the exception of 3' UTR where this proportion is around 60%.
It has generally been assumed that the steroid interaction/binding site is contained in an extracellular domain of the receptor, and that steroid interactions are of high potency, evidenced by the low aqueous ligand concentrations required to achieve potentiation of channel function.
An electrophoretic mobility shift assay confirmed that an oligonucleotide containing the binding site is recognized by DBP, but a point mutant version is not (Figure 7B).
More suggestions(17)
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