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The binding site is blanked with two loops, the gate and latch.
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Otherwise, this binding site is regarded as an incorrectly predicted binding site.
The occurrence of the Gal binding site is apparently independent from the binding profiles of GI NoVs.
Nonspecific binding sites were blocked for 1 h with 2.5% nonfat dry milk in TBS (10 mM Tris HCl, pH 7.4, 140 mM NaCl) and detection was performed for 2 h at room temperature with affinity-purified polyclonal anti-GbE antibodies (Blank, Kiger, et al. 2011), diluted 1 1,000 in 2.5% milk/TBS.
The lower binding site was found more constructive favorable for binding.
One method to reduce the presence of these information-poor binding sites is to apply stricter binding site conservation criteria.
The Ach-binding MfdA is mainly dependent on D34, while reserpine-binding site is more hydrophobic.
Potential binding sites were elucidated by docking.
Here, these binding sites are not discussed one by one.
These binding sites are highly charged.
Noteworthy, these binding sites are juxtaposed to GATA3-binding sites.
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