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It is made up of two homologous halves each possessing binding site for iron [ 4– 8].
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Since aTf has two binding sites for iron, we explored the effects of ferric citrate and ferrous sulfate at 2.5 μM, a concentration that equalled the amount of binding sites on the added aTf.
Another binding site for the iron-containing cofactor is most probably Cys176, which is located at the bottom of a deep intersubunit cleft and which has been shown to be essential for enzyme activity.
Iron is the binding site for ACC and oxygen.
The M1 subunit is the binding site for nucleotides and the second subunit, M2, is a metal binding site that requires both a non-haeme iron and a tyrosine-free radical for its activity (Smith and Karp, 2003).
The presence of a high affinity binding site for CIM on P450 in liver microsomes, with both the imidazole and cyano positions of CIM interacting with the hemin iron is well documented.
Adjacent to the iron of the cofactor modelled as a ligand to Cys176, an extended U-shaped extra electron density, interpreted as a polyethyleneglycol fragment, suggests a binding site for the substrate methenyltetrahydromethanopterin.
Potential binding site for the 3IVX was identified using PDBSUM.
In this case the binding of xylooligosaccharide to the active site of β-xylosidase creates a binding site for fructose.
Recent studies have investigated the repression of miR-210 on the iron sulfur cluster (ISC) assembly proteins, ISCU1 and ISCU2, whose 3′-untranslated region (UTR) is predicted to contain a highly conserved binding site for miR-210.
Gal4p has one binding site for Mig1p.
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