Each of the clusters are comprised of a specific number of LA-repeats and represent a putative ligand binding site for a diverse group of ligands; for instance, the second cluster binds ApoE-β-VLDL, lactoferrin, aprotinin, lipoprotein lipase, and receptor-associated protein (RAP) (Dolmer and Gettins, 2006; Orlando et al., 1997).
A structural alignment of CalU16 with these homologues highlights the striking conservation of the core structural fold, which contributes to the binding site for a structurally diverse array of ligands including steroids, lipids, hormones, and polyketides.
In addition to coding regions, Helitrons have mediated massive movement of a diverse collection of host sequences, including promoters, poly-A addition sites and binding sites for various regulatory proteins across the genome.
RPT5 appears to have a binding site for at least two diverse substrates, but the ultimate fate of each substrate differs.
However, it is very difficult to imagine that enzymes like CPO and CYP family have multiple binding sites for their diverse array of both their final substrates and initial activator (peroxide).
We present evidence and argue herein that it is highly unlikely that both CPO and the CYP family of enzymes (as exemplified by CYPs 2C9, 2E1 & 1A2) possess multiple binding sites for their diverse array of substrates.
Most VS methods suppose a unique binding site for the target, but it has been demonstrated that diverse ligands interact with unrelated parts of the target and many VS methods do not take into account this relevant fact.
In this case the binding of xylooligosaccharide to the active site of β-xylosidase creates a binding site for fructose.
