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Streptavidin protein is composed of four monomers, each containing a binding site for biotin.
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Therefore, the most likely binding site for biotin-ULS is the methionine in the HIS-tag of the recombinant protein.
Since the binding sites for biotin on avidin and streptavidin are pockets buried about 9 Å beneath the surface of the proteins, spacers can affect the accessibility of biotinylated compounds for efficiently binding avidin or streptavidin conjugates.
Although the lanes were equally spaced and uniform, and the grid thus appeared regular, the fluorescence intensity decreased slightly along the length of the lanes, indicating that the binding sites for biotin were not saturated.
Third, we asked if there was a binding site for the biotin regulatory protein BirA near the inserted genes [24].
Biotin carboxylase (PDB: 1bnc) is a homodimer with two binding sites: an active site in which the ligand biotin is bound and a binding site for the second identical protein subunit (Fig. 4A and B).
From structural studies of RePC and Staphylococcus aureus PC, the binding site for acetyl-CoA has been identified as an allosteric domain that is surrounded by the BC, CT, and biotin carboxyl carrier protein (BCCP) domains.
The biotin-cap-DOPE provided binding sites for streptavidin.
This amplification is due primarily to the multiple biotin molecules on the secondary antibody, which provide multiple binding sites for the streptavidin-label conjugates.
sharing binding sites for miRNAs.
> Multiple alignment of S. toxytricini PccB with other Streptomyces PccB and Mycobacterium AccD5 revealed the presence of acyl-CoA binding site and biotin binding site (Fig. 3a).
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