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We used Patser [31], [32] to scan this region for any potential binding site for any of the 263 mammalian transcription factors in TRANSFAC [33].
We used eight types of UniProt functional features: ACT_SITE: amino acid(s) involved in the activity of an enzyme BINDING: binding site for any chemical group (e.g. co-enzyme, prosthetic group, etc).
In this run, PhyloGibbs-MP searched for motifs in 500bp contiguous regions, separated on average by 500 bp (thus expecting that about half each region is occupied by cis-regulatory modules), and within each such region, on each sequence, the probability of a given site being a binding site (for any factor) is 0.005.
These additive effects come mainly from different miRNAs, since only a small fraction of all predicted targets contain more than one miRNA binding site for any single miRNA [ 34].
This motif, however, was not identified as the binding site for any known transcription factor, as deduced from searching the PLACE [ 14], TRANSFAC [ 15] and AGRIS [ 16] databases (not shown).
A locked glucose unit only facilitates the binding of the locking exo-R (exo-N); it does not constitute an available binding site for any other cellulases, nor can its glycosidic or hydrogen bonds be cleaved or broken, until the locking cellulase binds directly to it.
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Coupled with whole-genome DNA microarrays, ChIPs allow one to determine the entire spectrum of in vivo DNA binding sites for any given protein.
Assuming that we have access to the previous nucleotides before the start of the actual sequence, the likelihood of a sequence having no binding sites for any TF is, where.
It is challenging to rigorously assess subtle improvements due to the scarcity and unreliability of verified binding sites for any ChIP-seq dataset.
In many gene promoters it is possible to find multiple candidate binding sites for any particular transcription factor.
Only CNE 10 lacked binding sites for any of the twelve transcription factors in either species examined.
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