We compared the 389 binding sites described in the Gevry study to the 390 sites detected in the Carroll study and found only about 50% overlap (binding sites were considered the same when not more than 2000 bp apart, data not shown).
This would suggest the Mtb quinol-binding site described in our study is not reflective of either of two quinone sites described in the crystal structure of Feng et al. This study establishes that Mtb NDH-2 catalyzes quinone reduction through a two-site ping-pong mechanism, which is distinct from the sequential, ternary complex mechanism proposed for yeast homologue Ndil.
Alterations in the IPP binding site described above did not affect the position of RIS in the binding pocket in either mutant (Fig. 4A).
Present in this list are portions of sequences conforming to Su(H) binding sites described above.
In contrast, the truncated plectin produced by c.13458_13473dup harbors the IF-binding site described above.
It is possible that the loss of Zfp335 binding to the target sites described in our study may be unique to T lymphocytes, resulting in T cell-specific dysregulation; however, this hypothesis is not favored by preliminary evidence showing that target genes such as Ankle2 were also downregulated in B cells (data not shown).
Our two-hybrid analysis confirmed the L1 sub-domain of the SjIRs contains the insulin binding site, as described in HIR, SmIR-1, SmIR-2 and EmIR [25], [26].
ln p-value) cutoff valn p-valueredicutoffites were selected according to the values oforonfirmed functional binding sites as described in predictedsiteses (Hare et al. 2008a; Ho et al. 2009; Starr et al. 2011).
The nucleosome occupancy profiles around distal sites in both GM12878 and K562 cells were similar to those around all binding sites, as described in Figure 1A.
In addition to the two AP-1 binding sites previously described in the 5'LTR negative regulatory element (NRE) of different HIV-1 neurotropic strains [8], three AP-1 sites have been characterized by our laboratory downstream of the transcription start site in a large nucleosome-free region termed HS4 (nt 465-720), which functions as an enhancer towards HIV-1 5'LTR transcriptional activity [9].
