The proportionality factor between the protein-bound concentration and the concentration of the respective low-molecular-weight species is derived in a similar way, using the association constant K ijkl for the binding to the lth binding site, and assuming linear binding (i.e., a negligible loss of the binding site concentration on binding).
As experimental data, they used binding site information, concentration data of the TFs and the promoter-sequence for the investigated target genes.
Note that the same study found only one binding site of palmitate at protein concentration of 20 μM when fluorescence technique was used instead of ultrafiltration (Wang et al. 1998).
From the Gran function of the zinc sulfide suspension (see Figure 7), the equivalence point (Ve) of acid-base titration is decreased from 2.25 mL to 0.3 mL with increasing solid concentration" The proton binding site of the solid surface in the suspension is defined as Figure 7 Gran plots of zinc sulfide suspensions.
Figure 8 Surface proton binding site of zinc sulfide as a function of solid concentration.
The reason for the non-linear response at higher SOD1 concentrations is that the binding site of the gold nanoisland was nearly saturated with SOD1 protein.
One is the standard positional free energy required for bringing one Lac repressor molecule to its DNA binding site at 1 M concentration of Lac repressor.
With increasing the solid concentration from 0 to 2 g/L, the increase of pH buffer capacity of sample suspension indicates clearly the influence of the concentration of surface binding site and the acid-base properties of these sites.
We use the following notation: [ M] is the concentration of VEGF binding sites of the ECM, PBM or EBM (the location is denoted by the corresponding subscript, e.g., [ M ECM ]); [ V] represents the concentration of any unbound VEGF isoform (unless specified by a subscript, e.g., [ V 121 ]) in the available interstitial fluid; k on and k off the kinetic rates for binding and unbinding respectively.
