Furthermore, TLC1 with two extra Ku-binding sites causes a 10-fold increase in TERT enrichment at telomeres, and this increase in telomerase recruitment requires SIR4.
Tethering of mammalian Gld-2 to an mRNA using a fusion with an RNA binding domain and an mRNA with a binding site causes strong polyadenylation in immature Xenopus oocytes, indicating that the protein is constitutively active and only needs to be recruited to the mRNA to elongate the poly(A) tail [62].
A combination of electrophoretic mobility shift assay and RT-PCR were used to confirm that a predicted change in an SF2/ASF binding site caused exon skipping in the CFTR gene.
In silico analyses predicted a change in the binding of several transcription factors including the introduction of a Sp1-like binding site caused by the introduction of the -151 variant.
Using UV visible spectrophotometry, we show that the removal of K+ binding site causes substantial changes in spin states of both the ferric and ferrous forms.
The holo structure additionally reveals a binding site for fluoride ion, which we have shown to be an inhibitor of the enzyme in vitro, and suggests that the inhibition arises from the occlusion of the substrate binding site caused by the hydrated halide ion.
These relationships could be explained by various competitive interactions between metals for intracellular binding sites, causing, for instance, a displacement of lead from its renal store, as has been shown with both cadmium and mercury (Fowler 1998).
First, there are extreme differences in the information content among position weight matrices representing motifs of transcription factor binding sites, causing false-negative or false-positive matches [13].
Rilpivirine acts at the hydrophobic position near the NNRTI-binding site, causing inactivation of the reverse transcriptase enzyme, thus terminating DNA synthesis of the HIV virus.
(A ) Perturbation of kni and its binding sites cause full reporter expression between the stripes.
