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The oligonucleotide 5′-CUCAGGCUGGAGUGCAGUGGU-3′ of miR-1273g-3p's binding site can code the LRLECSG, SGWSAVV, and QAGVQW oligopeptides.
The oligonucleotide 5′-CACUGCAACCUCCAUCUCC-3′, in the miR-1273f binding site, can code the HCNLHL, TATSIS, and SLQPPS oligopeptides.
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The new binding site can be introduced with a minimum of four amino acid changes.
The value of n of BSA at all three studied temperatures is approximately equivalent to 1 as fractional binding sites don't occur and no < 1 binding site can be present suggesting only one binding site for rivaroxaban.
Each hormone binding site can be divided into an inner and outer binding cavity.
In addition, an individual domain or binding site can mediate binding to more than one protein.
Unlike many plant miRNA targets, which are almost completely complementary in open reading frames (ORFs) [ 18], the binding between animal miRNAs and their target sites has incomplete complementarity in base-pairing, and binding sites can be found in 3' UTRs, 5' UTRs and coding regions of target genes [ 19- 21].
As described immediately above, many potential energetically favorable binding sites can be found in 5'UTRs and coding regions, as well as in the 3'UTRs.
However, functional miRNA binding sites can also occur within the 5' UTR [8] or coding region [9].
This selection requires the presence of sequences within the target mRNA which are imperfectly complementary to the miRNA sequence; miRNA binding sites commonly occur within the 3'-untranslated region (UTR) of the mRNA, but functional miRNA binding sites can also occur with the 5'UTR [ 7] or coding region [ 8].
Individual binding sites can be observed via the green FitCons2 class 40 (ρ = 0.43).
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