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In our structure, sulfate ions are bound to the canonical binding site, but not to the proposed secondary site.
The SRY antibody obtained from Dr. Peter Koopman, Brisbane, Australia, pulled down the fragment of Tcf21 promoter containing the predicted SRY binding site, but not the negative control region without its binding site (Fig. 4C).
In accord, PP2 rescues activation of IL-8-LUCwt and IL-8-LUCmtAP-1 which harbor the intact NF-κB binding site, but not IL-8-LUCmtNF-κB in Thy-1+ MEFs (Figure 7).
Nevertheless, the results can be also explained if the mod mdg4) allele is a hypomorph that affects the insulator activity of the gypsy retrotransposon insulator, which has 12 copies of the Su(Hw) binding site, but not the function of endogenous insulators present in the Drosophila genome, which only contain 1 2 copies of this sequence.
Combretastatin A-4 interacts with tubulin at the colchicine binding site but not in the same pseudoirreversible manner.
1) "[S]liding off of target sites" implies a specific physical model in which Ago2 departs from its seed-matched binding site but not the RNA itself.
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As expected, only EB-MMG and EB-MMG_N, which have the putative EB1 binding sites, but not EB-MMG_C, which does not, were able to bind EB1 (Fig. 6A).
AliBaba2 program analysis revealed that there are only AP1 binding sites but not NF-κB binding sites existing in the promoter region of mouse amylin gene.
Anti-HA antibody pulled down the oligonucleotide with intact SRY binding sites, but not the one with mutated SRY binding sites (Fig. 4A).
In another test we show that our predicted autoregulatory binding sites cluster surprisingly close to the transcription start sites as expected for functional binding sites but not for falsely predicted ones.
Using our W-ChIPsmotifs program [43], [44], we identified a number of motifs that were highly enriched in the ZNF274 binding sites but not in the control set of C2H2 3' exons (Supplementary Table S4).
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binding step but not
binding affinity but not
binding reaction but not
binding domain but not
binding constraint but not
binding surface but not
binding pocket but not
binding ability but not
binding region but not
binding motif but not
binding protein but not
binding today but not
binding site but lacked
binding site but lacks
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