Sentence examples for binding site as previously from inspiring English sources

Interaction and colocalization of phosphoT130-cdc25C with Plk1 demonstrate in living cells, that the sequence around pT130 acts as a true Polo Box Domain (PBD) binding site as previously identified from in vitro peptide screening studies.

PCR was performed to amplify a SHIP-1 promoter fragment containing the Fli-1 binding site, as previously described.

Although the PTEN C124S mutant did not inhibit EMT or cell migration, we found that the lack of inhibition of EMT by the phosphatase-dead PTEN C124S protein could be partially recovered by mutation of the C-terminal phosphorylation sites (Table S1), which may result in the protein's unfolding and exposure of the C2 domain and C-terminal tail or PDZ binding site as previously proposed [5, 29].

The presence of the phenylpropyl moiety increased activity significantly against the enzyme (cf. 6 and 7; 5 and M), suggesting that the phenylpropyl moiety has an interaction in the binding site, as has been previously seen in the polyamine class of inhibitors.

It is thus likely that Ste11 regulates the majority of pheromone-regulated genes, perhaps in some cases via co-operative binding to a number of relatively weak binding sites as previously reported [ 14].

U700 may play a dual role in the kissing-loop by providing a flexible linker to SLV as well as a Mg2+-binding site, as previously proposed from kinetic studies of U700 variants.

A stiffening of Tm in Position M would enable free access to myosin binding sites, as has been modeled previously [3].

We then apply our motif scanning algorithm to predict putative CRP binding sites in the 12 cyanobacterial genomes that encode a crp gene using the profile of the 112 putative CRP binding sites as well as that of the previously prepared -10 like box from cyanobacteria [ 32].

Predicted motif substrates for EMSA were prepared by annealing biotinylated oligonucleotides containing the binding site as described previously [ 13].

It can also explain the inability of two DCS molecules to simultaneously occupy each binding site, as we have previously shown.

Briefly, sections were deparaffinized, rehydrated, and antigen retrieved before blocking of nonspecific binding sites, as detailed previously [28].