The C-terminal Gly and a l-amino acid analogue at the Cys binding site were necessary for inhibition, suggesting that human GGT was highly selective for glutathione (γ-Glu-l-Cys-Gly), whereas E. coli GGT are not selective for glutathione, but still retained the dipeptide (l-AA-Gly) binding site.
These results suggest that the p300-2 binding site is necessary for the basal and UV-induced tregulationofal regulation of MMP-1.
HKIII is primarily expressed in the lung, kidney, and liver [7], [9], [41], and an Oct-1 binding site is necessary for basal HKIII expression [9], but little else is known about the regulation of HKIII activity.
Importantly, mutations in the sequence targeted by miR-21 in RhoB 3'UTR reduced the observed down-regulation of luciferase activity by pre-miR-21 validating that this predicted binding site is necessary for miR-21 dependent RhoB expression (Figure 5B).
Taken together these results show that tau phosphorylation at the Ser262 MT binding site is necessary for Wnt5a evoked axon outgrowth and growth cone repulsion.
Since the Meis1 binding site is necessary for CR4.2-GFP expression, we confirmed the expression of Meis1 protein in CR4.2-GFP+ cells using immunohistochemistry (Fig. 6).
Functional analysis of MDA-MB-231 cells expressing mutated S100A7 confirmed that the Jab1 binding site is necessary for the effect of S100A7 on NF-κB, phospho-Akt, and promotion of tumorigenesis in vivo [ 39].
By utilizing novel time lapse in vivo imaging techniques (Rana et al., 2005) it was shown that the NFAT binding site was necessary for the suppression of the fast troponin I promoter by a slow stimulation pattern, while the destruction of the site had no effect on the positive regulation of this gene by fast activity (Rana et al., 2008).
19 It can be used as a phasing tool and only a few binding sites are necessary, whereas mononuclear heavy atoms must bind to multiple sites to provide useful phases, especially for large proteins.
In Xenopus embryos adjacent Pum1 and CUGBP1 binding sites are necessary to achieve spatially regulated translation of the xCR1 mRNA [50].
