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These weaker interactions with the second binding site are consistent with the negative cooperativity between these sites that is observed with T4 (Ferguson et al. 1975).
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This location for D2 adjacent the pABA binding site is consistent with the presence of sulfonamide resistance mutations within this loop region.
DOI: http://dx.doi.org/10.7554/eLife.03080.016 The identified binding site is consistent with mutations of Arg149 and Arg150 of uS11 in Chinese hamster ovary (CHO) cells that have been found to confer resistance to emetine (Madjar et al., 1982).
The involvement of the carboxylate of 6 in strong polar interactions within the binding site is consistent with known SAR in this series: The inactivity of ester prodrug 7 and its congeners is indicative of the need for a free carboxylate.
The resulting curvilinear plots (Figure 1G and Figure 3) are characteristic of cells expressing both high- and low-affinity EGF receptors, and the ratio and affinities of these binding sites are consistent with previous reports [5].
By examining the conservation of binding sites identified in Saccharomyces cerevisiae, we found that while some transcription factor's binding sites are consistent with a model of binding site turnover, a quarter of the transcription factors are consistent with some amount of regulatory divergence.
The overall correlation in expression, and differences in correlation (although small) between alleles that differ in the number of putative binding sites, are consistent with a model wherein differences in Abd-B expression contribute to differences in activation of Men expression in trans (see the section Discussion).
The distribution of 125I-PACAP27 binding sites was consistent with that of PAC1-R and VPAC1-R mRNA [52].
The presence of both ferrihydrite and goethite binding sites is consistent with the X-ray diffraction results, which indicate that the nanoparticle suspensions exhibit features consistent with both ferrihydrite and goethite (Figure 1).
This low sequence conservation at the binding sites is consistent with our experience with the majority of transcription factors.
First, as shown here, the presence of independent binding sites is consistent with a two-site ping-pong mechanism.
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