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Specific anti-PnPS IgG serum concentrations were measured using VaccZymeTM Anti-PCP IgG Enzyme Immunoassay kit from Binding Site (The Binding Site) according to the manufacturer's instructions.
There are three other miRNAs miR-34a, 449a and b that are predicted to bind to the same binding site according to the TargetScan.
In addition, conformational changes of this RNA binding loop can change the nature and location of the optimal ligand binding site, according to ligand binding site prediction results.
For each gene we plotted the strength of its strongest upstream Met4 binding site according to the model in Fig. 5 and its average expression change for induction and repression experiments (Fig. 7).
The Ca2+ shuttling mechanism was observed in simulations of Calbindin D9k where the main Ca2+ attractor is the side chain carboxylate of E60, which is not a part of the binding site, according to experimental structures.
Bacterial CDOs show low overall similarity with eukaryotic CDO (12 21% identity with rat CDO) but have a conserved 3-His metal binding site, according to sequence alignments.
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Conversely, software called Binding Site Match Maker was developed, which could align binding sites according to their similar physiochemical properties and generate common sites.
We selected candidate ER binding sites according to the results of a published genome-wide analysis [36] and confirmed the binding of ERα to ER target genes including MYB, JMJD2B, and GREB1 (Figure 5A).
An additional parameter addresses the fraction of the top binding sites according to the internal scores provided by each MDB.
cPutative Zur binding sites according to MEME-SUITE and FIMO analysis on the basis of MAP K-10 genome [NCBI NC_002944].
In this case, a population may accrue binding sites according to the mutational bias, implementing the same pathway function with either many or few binding sites.
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