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Binding signals were visualized with TMB substrate.
Binding signals were visualized using o-phenylenediamine dihydrochloride (OPD) substrate and the light absorbance was measured with an ELISA reader at 492 nm.
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Antibody binding was then detected using chemiluminescence (Cell Signaling Technology, Danvers, MA) and signals were visualized by autoradiography.
The signals were visualized by PhosphorImager.
Radioactive signals were visualized and quantified by Phosphoimager (Bio-Rad).
Signals were visualized using ECL Plus reagents (Amersham Pharmacia Biosciences).
Signals were visualized with the enhanced chemiluminescent (ECL -reagent (PJK).
The immunoblot signals were visualized using enhanced chemiluminescence (ECL).
Immunorreactive signals were visualized by ECL Plus (GE Healthcare).
Signals were visualized with ECL plus Western Blotting Detection System (Amersham Biosciences, UK).
Signals were visualized using a chemiluminescent ECL substrate (GE Healthcare).
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