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Binding signals were not detected when the control buffers (i.e., no SsoRadA protein) were injected.
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Binding signals were corrected for nonspecific binding by subtracting the flow cell-1 signal.
Binding signals were visualized with TMB substrate.
Signals are not looking good.
Check that signals aren't conflicting.
The effect of this differential binding on TNF-α signaling is not clear.
Mec1p binding is not detected at this time, suggesting that Cdc13p prevents RPA binding so that Mec1p-mediated DNA damage signaling is not elicited by the telomeric single-stranded G tails.
The signal was not great.
Figure 4A shows the results obtained for α-actinin 1-GNE versus LDH-B-GNE interactions when tested at the same conditions: a binding signal was obtained with α-actinin 1, but not with LDH-B.
Either way, the signal isn't good.
Our results showed that all DARPins tested induced strong signal suppression of more than 50%% (Fig. 1), even when using only 25 pM (monomer) VEGF-A, whereas the isotype controls (i.e. non-binding DARPins) were not affecting the signals.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com