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As shown on Figure 2A (lanes 3, 4, 5, 6, 8) for PGK, PGK-FL1 to FL3 and PGK-FL5 truncations, significant binding signals located at approximately 42 KDa corresponding to actin molecular mass were revealed by anti-His MAb.
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The XRD signals located at around 31.5° and 50.3° ascribed to tetragonal ZrO2 phase.
The NMR signal located at 78 to 82 ppm is also asymmetric in shape.
The 1B8 clone represents mAb having a binding site located at the TSP3 domain, which produced a specific signal with pCOMP, mCOMP, EGF1-TIII8, TIII1-CG and TIII1-8 fragments, but produced no signal with EGF1-4 and CG fragments.
These data together suggest that NLX binds with high affinity to FLNA with the binding site located at FLNA2561 2565.
The 15A11 clone represents mAb with a binding site located at the EGF-like repeat domain, which produced a specific signal with pCOMP, mCOMP, EGF1-TIII8 and EGF1-4 fragments but produced no signal with TIII1-8 and CG fragments.
ECM components signal by binding to integrins located at the cell membrane.
In addition, a completely buried negatively charged glutamate, expected to be critical for drug binding, is located at the center of the binding pocket.
In GLUT1, Walker B nucleotide binding domain located at the cytoplasmic loop between TM8 and TM9 has been shown to play a role in ATP binding [38].
(27) One is the gp16 binding domain, located at the 5′/3′ paired helical region.
TTR has two T4 binding sites located at the dimer dimer interface.
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