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A second cis-Golgi binding signal is present in the C-terminal domain of GMAP210.
In the dataset for IRF1, a weak binding signal is present across the promoter region and a stronger broad peak is overlapping with the NF-κB binding region in the proximity of TSS.
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This zeroing happens when the binding site numbers at the leaves of the tree are too similar and thus no phylogenetic signal is present in the data.
These findings demonstrate that STAT3 signalling is present in prostate carcinoma cell lines in response to EGFR signalling and that this signalling pathway increases STAT3 binding to target.
A detailed discussion on the ligand-receptor binding, trafficking and signaling is presented in [44], where the explicit expression for ligand-receptor binding action in the case of uniformly distributed ligands around the receptor location is provided.
Binding signals were corrected for nonspecific binding by subtracting the flow cell-1 signal.
After subtracting the reference spot signal, resulting binding signals were fitted.
Binding signals were visualized with TMB substrate.
Hybridization signals are presented in Figure 2.
We reasoned that the high abundance of hemoglobin might interrupt the target mRNA binding, or contribute to pseudo-binding (a nonspecific, background signal that is present in the absence of any significant sequence similarity) to the probe, and, therefore, distort the true expression signal.
In the RELA gene promoter, a strong binding signal for NF-κB is present in 9 out of 10 cell lines, 5 of which are defined peaks and are mostly consistent with the binding sites computationally located in the region further upstream from TSS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com