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Thus, to scan the cell surface targets for Gal-4 on T cells and specify the exact binding side, we performed immunoprecipitation for Gal-4, followed by immunoblotting for CD3, CD7, and integrin-β1.
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To test the binding specificities, we performed ITC to measure the equilibrium disassociation constants (Kd).
To test whether SVOP is a nucleotide binding protein, we performed similar photoaffinity labeling experiments.
To identify if the present GmPHDs has any DNA binding activity, we performed gel-shift analysis.
To further probe for HNF6 binding activity we performed EMSA band shift assays.
To demonstrate that this binding is specific, we performed a competition binding experiment.
To assess the impact of the T109M mutation on PFN1 binding to PLP, we performed molecular-dynamic simulations.
My focus is to add something to the side, and we performed really well.
"It's about everyone taking responsibility and we're a better side than we performed today.
To determine whether β-casein 197 had DNA-binding capabilities, we performed a gel retardation assay.
To determine whether GAG-binding capacity is required for receptor binding and cell activation, we performed competition radioligand binding and calcium mobilization experiments using one of the non-heparin-binding mutants, R46A.
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