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First, four AP-2 binding sequences were identified in the ERBB2 promoter [ 12- 15].
ATAF2 binding sequences were identified via an immuno-pull-down assay using purified hexa-histidine tagged ATAF2 and genomic DNA isolated from Arabidopsis thaliana ecotype Shahdara.
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Moreover, both ZNF644 and WIZ-binding sequences were identified at the loci co-occupied by ZNF644 and WIZ, such as CWH43, DIP2C, and ROCK1 loci.
NF-κB-binding sequences were identified by TRANSFAC 4.2 filtering matrix scores by minimizing the sum the false positive and negative error rates.
Three putative heparin-binding sequences were identified within the CRD-spacer sequence of ADAMTS-4, one within the CRD and two within the spacer, and peptides corresponding to these sequences were shown to inhibit the binding of ADAMTS-4 to heparin [ 9].
A well-conserved consensus Sox binding sequence was identified in cyclin D1 proximal promoter, located at approximately −74 bp relative to exon 1 in the human and mouse genomic sequences (Fig. 6C).
p53 binding sequence was identified in mtDNA suggested that p53 might be involved in the regulation of mitochondrial transcription and replication (Heyne et al, 2004).
A conserved C/EBP α binding sequence was identified in the core promoter region of the FTO gene, suggesting that the binding sites may play important roles in regulating the expression of FTO gene.
(J ) Both ZNF644 and WIZ-binding sequences are identified at CWH43, DIP2C and ROCK1 loci, which are co-occupied by ZNF644 and WIZ.
Binding reactions employed synthetic peptides mimicking a putative HSP72 binding motif of N. Sequences were identified that bound HSP72 with affinities comparable to well-characterized activity control reactions.
Potential transcription factor binding sites within the leader mRNA sequences were identified using two prediction programs.
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