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Theoretical DFT calculations were used to explain metal binding capability of tpmc ligand and the binding sequences were found to follow the order: Cu2+ ≫ Ni2+ > Co2+ > Zn2+.
Furthermore, the binding sequences were found in the upstream region of other genes analyzed in Figure 6 (data not shown).
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CARGAT and WUS binding sequence were found in only 6 and 18 of those respectively.
Interestingly, human- and primate-specific NANOG-binding sequences were found to be located near hESC enhancers, whereas NANOG-binding sites common with rodents were located next to the transcription start site of protein-coding genes (fig. 5 A and supplementary fig. S7, Supplementary Material online).
It is interesting to note that AGL2 binding sequence was found in no other promoter region of class III peroxidase genes AtPrx30 and AtPrx55.
An Ets binding sequence was found spanning from -70 to -61 nucleotides (ACCGGACGCC) in the promoter region of the NmeGp1 gene from sponge A. queenslandica and from -365 to -374 nucleotides (ACAGGATATA) in the promoter region of the NmeGp1Sd gene from sponge S. domuncula.
Using the version of the known motif found by MEME and the corresponding unmasked sequences, the positions of the binding sites within these sequences were found by MAST, a companion tool of MEME.
Interestingly, the relative binding affinities and specificities of the TA sequences were found to correlate with the energy of the structures predicted by MFOLD [34] (Table S1).
Immunogenic sequences were found clustered in the RNA binding domains of A2/RA33.
In the E83 group 80 of the 83 input sequences were found to have a NF κB binding sequence, with 21 of the 22 known targets being found.
After three iterations, twelve sequences were found with strong similarities to the sequence of human B-block binding subunit.
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