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To measure the similarity between binding sequences of a pair of factors we assessed the distances between their PSSMs.
This result suggests that the crystallographic structure of a protein-DNA complex indeed contains enough information to locate the binding sequences of a protein.
sSr is defined as where Ns is the number of sequences having their motifs correctly predicted, and N is the total number of binding sequences of a transcription factor.
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Models of the complexes formed by interaction of JAZ ZF2 (homology modeled using the Phyre webserver), JAZ ZF3, and ZFa ZF1 with a minimal binding sequence of A-form dsRNA (determined during the NMR experiments to be 19 base pairs) were generated using HADDOCK version 1.2.
Future work based on co-crystal structure studies with a synthetic peptide, containing the 564 VIFYLRDK 571 sequence and PP1c, will be required to determine the binding sequence of a FxxR/KxR/K motif on PP1c.
Potential PWMs which corresponded to the binding sequence of a specific transcription factor were selected based on the binding affinity within DHS sites in the gene promoter region, as detailed in the methods.
The consensus sequence TCCNNNGAA of the IFN-γ-activated site (GAS [13]) does not comprehensively describe all binding sequences of STAT1, but it allows an unbiased assessment of the positional precision of the determination of STAT1 binding in sequence sets.
The score S (M; {n}) of a putative binding sequence of L nucleotides, (n1,..,nL,nL), for a particular motif M is the log-likelihood score calculated from the motif's PSSM.
Based on structural evidence a binding sequence of NADH, L-arginine and pyruvate leading to the active complex was suggested [10].
By searching databases with the ALG-2 binding sequence of AIP1/Alix in combination with an ER localization signature we found Scotin.
For instance, the binding sequence of tobacco (Nicotiana tabacum) NtWRKY12 with a WRKYGKK motif is TTTTCCAC, which deviates significantly from W-box [ 76].
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