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Furthermore, the miR-10a binding sequences in the BCL6 3′-UTR were highly conserved across species.
Moreover, the miR-203 binding sequences in the Slug 3′-UTR are highly conserved across species.
Furthermore, two of the three miR-29b binding sequences in the CDK6 3′-UTR are highly conserved across species.
Furthermore, the miR-495 binding sequences in the 3′-UTR of JAM-A mRNA are highly conserved across species.
Moreover, there was perfect base-pairing between the seed regions (The core sequences that encompass the first 2 8 bases of the mature miRNA) and cognate targets, and the miR-19a/b binding sequences in the MTUS1 3′-UTR were highly conserved across species.
The DNA excess should select for preferred histone octamer binding sequences in the absence of epigenetic factors and minimize the possibility that a nucleosome positioned by sequence does not serve to position adjacent nucleosomes by a sequence-independent boundary effect.
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Since a previous report identified the presence of a STAT3 binding sequence in the BST-2 promoter22, and IL-27 and IL-6 both induce STAT3 activation27, we reasoned that, like IL-27, IL-6 might enhance BST-2 expression.
A major unresolved issue is how transcription factors find their specific DNA binding sequence in the vast expanse of the cell and how they do so at rates that appear faster than the diffusion limit.
There was perfect base-pairing between the seed region (the core sequence that encompasses the first 2 8 bases of the mature miRNA) and the cognate target, and the miR-181b binding sequence in the PDCD4 3′-UTR was highly conserved across species (Fig. 2A).
Many prediction algorithms take into account the conservation of the microRNA binding sequence in the mRNA target.
The predicted NF-κB binding sequence in the human PIK3CA promoter was further tested using gel shift assay.
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