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Clathrin recruitment by AP2 is mediated by clathrin binding sequences in both hemi-complexes.
In particular, we identified several potential repressor binding sequences in both minimal promoters and showed that KLF15 can repress the activity of P1 promoter region.
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As shown in Fig. 4A, there was a dramatic loss of binding to the survivin promoter STAT3 binding sequence in both the canine OSA8 line and human SJSA line after exposure to SU6656 compared to cells treated with DMSO alone.
Furthermore, the effectiveness of the newly designed chimeric decoy ODN, which contains both Smad and Sp1 binding sequences in one decoy molecule (Smad/Sp1 chi decoy ODN), was demonstrated.
The various vinculin binding sequences in talin are similar [3] and the corresponding peptides all bind tightly to vinculin in vitro.
Continuing the work in budding yeast, it turns out that binding sequences in cells are strategically blocked or exposed in order to achieve quantitatively precise gene responses.
To identify putative FRα binding sequences in Hes1 and Fgfr4 promoters, 32P-labeled oligonucleotides were made from appropriate promoter regions and EMSA was performed using affinity-purified GST-FRα fusion protein (Fig. 3b, c).
From an evolutionary standpoint, it suggests that cells are able to modify a gene's point of activation or its expression capacity simply by mutating DNA binding sequences in either un-spooled or spooled segments.
Furthermore, the miR-10a binding sequences in the BCL6 3′-UTR were highly conserved across species.
Non-specific CLIP was then performed to identify the RBM24 binding sequences in HCV RNA.
Moreover, the miR-203 binding sequences in the Slug 3′-UTR are highly conserved across species.
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