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Several of these genes also contain a consensus Meis binding sequence within the 40 nucleotides surrounding the Hox/Pbx binding site.
Peptide truncation experiments showed FEI to be the minimal binding sequence within the DLFEIFEIER sequence, and adhesion experiments with peptides that include both the RGD and FEI sequences demonstrate that the two peptides bind synergistically to the receptor.
The reduced transcriptional activation of these genes in the absence of ATAF2 indicates that ATAF2 contributes to the transcriptional regulation of these genes, further confirming the importance of the identified ATAF2 binding sequence within the promoters of these genes.
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We chose the other sites based on potential SOX9 binding sequences within the peaks of hybridization.
Observation of the correlation between the sequence composition of the biosensor and the P1 strand of the ribozyme domain, as well as of their binding sequences within the substrate, with the cleavage efficiency prompts several conclusions.
The potential miR-188 binding sequences within the 3′UTRs of target genes were predicted by FindTar.
miR-200b/200c/429 expression consistently downregulates CRKL via predicted binding sequences within the 3′-UTR of the CRKL gene.
Interestingly, ischemia increases the levels of GLUT-1 in the brain via Sp1, a transcription factor that has specific binding sequences within the GLUT-1 gene (Santalucia et al., 1999) as well as within the EGF-R promoter (Nishi et al., 2002).
A putative p53-binding sequence within the CD133 promoter is essential for p53-binding and the subsequent recruitment of HDAC1 to facilitate epigenetic modifications of the CD133 promoter.
The DNA fragments that were immunoprecipitated with the anti-Rbpj antibody were PCR-amplified with eight pairs of primers flanking the potential Rbpj-binding sequence within the regulatory region of Mmp2.
Comparing nucleotide frequencies at each site of the SP1 binding sequence within its functional region, we found that the classical GC box 6-mer consensus was the preferred binding sequence in all eutherian mammals as well as in birds (chicken and zebrafinch).
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