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For gel retardation the following double-stranded oligonucleotide, corresponding to the NF-κB binding sequence, was used: forward-5'-GCCTGGGAAAGTCCCCTCAACT-3' (Invitrogen, CA, USA) was used.
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In the GAL4-based MATCHMAKER two-hybrid system, either the intact GAL1 UAS, which contains four GAL4-binding sites, or an artificially constructed UAS consisting of three copies of the 17-mer consensus binding sequence is used.
The TRANSFAC database, which is a collection of experimentally verified transcription factor and binding sequences, was used to verify the 28 motifs.
The construct with mutated fragment of the 3′-UTR of Drd1 mRNA without the putative miR-382 binding sequences was used as the mutated control.
Keywords "PPRE", "PPAR consensus sequences" "PPAR binding sequences" were used to search for literatures in pubmed and Google Scholar.
HEK 293 T cells expressing other nonselecting CLL BCRs with different antigen-binding sequences were used as controls.
For expression of recombinant proteins, each pET-5b vector containing the appropriate odorant-binding protein (OBP) sequence was used to transform BL21 DE3 pLysS and BL21 DE3 Rosetta-gami E. coli cells, for OBL21 DE3 Rosetta-gamitivE.y.
In this work a bi-functional recombinant protein, containing a starch binding module (SBM) and RGD sequence was used to functionalize a dextrin-based hydrogel.
Likewise, a recombinant protein containing a starch-binding module (SBM) and a RGD sequence was used in this work.
The relative position of the SD sequence in chloroplast mRNAs is an indicator of whether or not the SD sequence is used for ribosome binding.
Following binding selections to isolate functional clones, DNA sequencing was used to determine the wild-type/mutant ratios at each varied position, and these ratios were used to assess the contributions of each side-chain to antigen binding.
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