Sentence examples for binding sequence was mutated from inspiring English sources
This interaction was abolished when the NF-κB binding sequence was mutated (lane 6) or out-competed by adding excess unlabeled probe (lane 7), indicating that the interaction is sequence-specific.
In vivo tunability was achieved via an informed trial-and-error process: the wild-type L7Ae binding sequence was mutated with the intent of reducing the repressive strength of the interaction to varying degrees compared to the wild-type interaction.
Further when this inverted repeat sequence was mutated, SOX9 binding was abolished and the mutated Chimera B did not show any activity (Figure S7).
However, UAC UA AC from the forth Gomafu repeat showed decreased binding affinity with SF1 when the sequence was mutated to ynC Ur Ay or y Un Ay (Fig. 2D), even though the critical adenosine and uridine residues were present.
The SRE sequence was mutated from 5'-ATCACCCCAC-3' to 5'-ATCACGGCTC-3' (mutations underlined), previously shown to prevent SREBP-2 binding [49].
When this sequence was mutated as in 5′-AACCCTTATTCCCCTTTGCGTGAAG-3′ there was no shift.
When the signal sequence was mutated, occupancy was lost.
To test binding specificity, sequences that interacted with the miR-181b seed sequence were mutated from GAATGT to CTTACA, and the synthetic PDCD4 3′-UTR mutant fragment was inserted into an equivalent reporter plasmid.
To test the binding specificity, the sequences that interact with the miR-495 seed sequence were mutated (from GUUUGUU to CAAACAA), and the mutant JAM-A 3′-UTR was inserted into an equivalent luciferase reporter plasmid.
To test the binding specificity, the sequences that interacted with the miR-10a seed sequence were mutated (from CAGGGTT to GTCCCAA), and the mutant BCL6 3′-UTR was inserted into an equivalent luciferase reporter.
To test the binding specificity, the sequences that interacted with the miR-141 seed sequence were mutated (from AGUGUU to UCACAA), and the mutant HNF-3β 3′-UTR was inserted into an equivalent luciferase reporter.
