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The Ku binding sequence from the Glucokinase (GCK) gene promoter was used as a positive control for Ku proteins binding [ 37].
Removal of the putative binding sequence from the LRP6 3′-UTR construct abolished the inhibition of the luciferase activity by miR-30e overexpression.
We made constructs with or without the potential OCT4 binding sequence from the promoter region of p63 (Supplementary Figure S5a) and examined the effects of the potential binding sequence on the transcriptional activity of p63 using a luciferase promoter assay.
In the initial FASAY experiments the vector used has three copies of the p53 consensus binding sequence from the ribosomal gene cluster (RGC) immediately upstream of this minimal promoter.
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If we think of sequences S as vectors in a vector space V and H = { x ∈ V| ε x = -1} a hyperplane separating the binding sequences from the non-binding ones, then H is "supported" by those observed sequences S(a ), which corresponds to non-trivial γ a.
For comparative purposes, a consensus MYB-binding sequence from the bcl2 gene was used as a positive control [18].
While our physiological and microarray analyses demonstrated that ArcA of S. oneidensis may differ substantially from the canonical Arc system in terms of both functions and regulatees, the consensus ArcA-P binding sequences from these two microorganism are alike.
We derive a single refined probabilistic model of STAT1 binding sequences from these ChIP-seq data.
Experiments combining pull-down, cell penetration and apoptosis analyses in distinct human cells indicate that the PP2A1 binding sequence from Vpr77 92 is a new cell penetrating apoptotic sequence.
The most significant binding peaks (FDR ≤ 0.05), which coincided in both replicates, were analyzed by the program RSAT dyad-analysis to predict binding sequence from all peak genomic binding sites [ 36].
To explore the potential of collagen silk chimera for regulating biological activity, integrin (Int) and fibronectin (Fn) binding sequences from mammalian collagens were introduced into the bacterial collagen domain.
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